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41 In fact, co-detection of multiple viruses in respiratory infections has been increasingly frequent as a result of the availability of more accurate detection assays, and the development of microarrays and multiplex detection assays.
The association between a positive brain-reactive B cell response in the blood in direct ex vivo assays and the development of a consecutive relapse as shown for one case in Figure 3 suggests that the presence of brain-reactive B cells in the blood could be linked to a higher relapse rate.
Knowledge of the association between aberrations in apoptotic mechanisms and human pathology hopefully will be implemented for the design of improved diagnostic and prognostic assays and the development of novel, more efficient, therapeutic strategies.
The integration with other techniques, the fabrication of devices for certain biological assays, and the development of point-of-need diagnosis devices is predicted to be future trend for impedance sensing techniques.
The nature of various assays and the development of international standard reagents for qualitative and quantitative assessment of assay performance are outlined.
Integration of QSAR and in vitro methods is examined in the context of assessing mechanistic competence and improving the design of in vitro assays and the development of prediction models.
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In addition, we showed that more than 80% of RT-PCR confirmed H1N1-2009 caseroconvertvert using hemagglutination inhibition and virus microneutralization assays, and demonstrated the development of cross-reactive antibodies to other influenza A strains following H1N1-2009 infection.
In hematopoietic cells, overexpression of HOXA1 blocked differentiation, leading to transformation by colony formation in soft agar assays and to the development of acute myeloblastic leukemia in lethally irradiated mice [ 21].
Measurements included the characterization of viral proteins and genomic RNA in a single virion using dSTORM, the development of a GFP fusion assay, and the development of a pulse-chase assay for viral RNA production that allowed for the detection of both initial viral RNA and nascent RNA production at designated times postinfection.
Improvements in sensitivity of the assay and the development of algorithms to reduce noise have confirmed the chemical signature of SD waves in patients and shown that repetition of the events can drive down the local glucose concentration, to a level where the tissue is no longer viable.
While the assessment is purely computational and predictive in nature, it can guide further experimental efforts (e.g., detection and immunogenicity assays) and ultimately the development of effective and safe biotherapeutics for human use.
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