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RMFP assisted in the development of ELISA assays and preparation of manuscript.
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K. S. contributed to experimental design, performed experiments (with exception of IP accumulation assay), and preparation of manuscript.
The methods for the assay and preparation of Xyl-α1,3-GlcA-β-MU were described previously (Inamori et al. 2012).
The detailed protocol of the Rac pull-down assay and preparation of lysis/wash buffers was provided by Upstate Biotechnology (Rac activity Assay kit).
This is somewhat higher than a previous study (Souttou et al, 1998), most likely due to modifications of the ELISA assay and the collection and preparation of blood samples.
CP assisted with in vivo experiments and performed the ELISA assays, RB developed and assisted with the proliferation assays, AS helped in experimental design and preparation of the manuscript, PB co-designed the experiments and co-wrote the manuscript.
Samples were withdrawn for various analytical assays and for preparation of inocula for the SSCF experiments.
KKW was responsible for 3D gel preparation, capillary assays, sectioning, staining, data analysis, interpretation, and preparation of manuscript.
BS carried out the oxLDL assay and participated in data analysis and preparation of the manuscript.
For RNAse protection assays, preparation of total cellular RNA and detection of specific transcripts by RNAse protection analysis was performed as previously described.
This included solvents and materials for the delivery of substances to the assay, preparation of environmental and epidemiological samples, and methods for the concentration and determination of doses for testing gases.
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