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However, when one turns to the development a highly specific assays aiming at the replacement of animals, when determining whether a new chemical exhibits irritative and/or sensitising properties, no company can reasonably afford relying on one unique producer of skin or epidermal models.
The cold chain dependency for Capillus assay coupled with availability on the market, of newer HIV rapid tests that are cheaper, do not require cold storage and have acceptable performance characteristics compelled the Ministry of Health and Social Welfare to embark on evaluation of some of these rapid HIV assays aiming at developing alternative algorithms for use in Tanzania.
Although Mg2+ is constantly present at high concentrations in muscles and is therefore an important component that is customarily used in mechanical measurements, most of the in vitro assays aiming at quantifying the binding affinities of TnI fragments have been conducted in the absence of this cation.
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The enterocin-producing E. faecium (Microbial Type Culture Collection Center (MTCC5153)) was used as a positive control in assays aimed at detecting bacteriocin production.
There is a need to develop affordable, systematic, short-term in vitro assays aimed at allowing rapid assessment of potential toxicity.
The specificity of this multiplex was validated by comparison to that of other molecular identification assays aimed at Xap, on a wide collection of reference strains.
The high biological relevance of the latter modified bases explains why major efforts have been devoted to the design of a broad range of assays aimed at measuring globally or at the single base resolution, 5-hydroxymethylcytosine and the two other oxidation products in the DNA of cells and tissues.
Aggregation assays aimed at investigating the effect of ANS binding on the CLA of PDC-109 are shown in Fig. 4A.
Two other studies have documented high sensitivity of RT-qPCR assays aimed at detecting HIV-1, with a limit of 5 copies per 'reaction' and 1 copy/ml respectively [25], [26].
This retrovirally-based expression library was used in two independent unbiased genetic selection assays aimed at overcoming cell death induced by the chemotherapeutic 5-FU or the cytokine TNF-α.
To directly correlate Rv1208 and glucosyl-3-phosphoglycerate synthase activity to the biosynthesis of MGLPs in mycobacterial cells, whole cell lysates prepared from the wild-type and mutant strains of M. smegmatis provided enzyme sources in assays aimed at monitoring the formation of glucosylglycerate and MGLP precursors in vitro.
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