The procedure of DPPH radical scavenging assay adopted for the antioxidant evaluation of the four active components is efficient and reliable.
This assay, adopted from Kienzle et al [35] measures by flow cytometry the lytic activity of effector cells by counting the number of viable GFP+ target cells (as determined by propidium iodide exclusion) that were left after incubation with the NK effectors.
An assay adopted from Vicogne et al. [ 41] was used.
In vitro assays of the metastatic behaviour of melanoma cell lines have yielded contrasting data, dependent on the assay adopted [ 77].
The high throughput micro assay adopted in this study is based on Chundawat et al. [ 11], a procedure which has been standardized for solid delivery in biomass slurries, mass transfer related parameters, reproducibility and validity through comparision to conventional National Renewable Energy Laboratory protocols [ 12].
Then the standard protocol of MTT assays was adopted to determine the cell viability by SpectraMax M2e Microplate Reader (Molecular Devices, Menlo Park, USA).
Immunoblotting, real-time PCR, immunofluorescence and immunohistochemistry assays were adopted to examine HRH4 expression in case-matched CRC samples (n = 107) and adjacent normal tissues (ANTs).
In these two assays, the adopted physical posture was the same; however, the intention, mind focus, or the achievement of a "special mental state of awareness" was completely different.
A five-level-three-factor CCRD, including six replicates at the center point, totaling 20 assays, was adopted to evaluate the effects of enzyme dosage, molar ratio of methanol to oil and water content on the FAME yield of the reaction in this study.
The prices of the individual and overall 12 products were assayed by adopting a weighted mean price, which were calculated by dividing market value by market volume.
