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Alternatively for the xenogeneic suppression assays, a total of 0.4×106 splenocytes isolated from naive mice were co-cultured with 0.6×106 irradiated (3500 Rad) xenogeneic third party Lewis or donor Sprague Dawley splenocytes.
Permeability assays: A total of 100 μg of FITC-dextran (Sigma FD-20S) was dispensed into the upper chamber media.
Counting replicate assays, a total of 200 Affymetrix 44K assays and 184 Illumina 1536 assays are used.
From 1,148,942 assays, a total of 13,520,737 activity records originated, as reported in Table 1.
Tumor cell extravasation assays: A total of 4 × 10 TCs freshly labeled with CellTracker™ Red CMPTX (Invitrogen C34552: ex/em = 577/602 nm) were seeded into each insert.
To examine proteins from transient transfection assays, a total of 3 × 10 μl of cell extract from each triplicate was pooled for Western analysis.
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To evaluate the genotyping assay, a total of 92 HPV positive samples were tested in this study.
Moreover, in the context of HLA region genotyping using Illumina GoldenGate assay, a total of 11 SNPs within PSORS1 gene were available in our data for 1203 subjects.
We assayed a total of 346 blood samples from 301 individuals collected from 1984 2004, comparing hemoparasite levels during CDV epidemics with typical infection levels in these populations.
Recently, two large-scale selection screens in maize assayed a total of 1869 genes for evidence that they were targets of selection during maize domestication or improvement [5], [6].
At 4 h and 18 h following infection, the culture medium was collected and analyzed for cytokine/chemokine content using dedicated antibody arrays, which could assay a total of 65 cytokines, chemokines, growth factors and other soluble immune mediators.
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