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Inhibin B was assayed with the use of Gen II ELISA (Immunotech) on a Beckman Coulter analyzer.
FSH was assayed with the use of electrochemiluminescence immunoassay designed for use on an Elecys and Cobas immunoassay analyzer (Roche Diagnostics).
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Exons 18 24 encoding the tyrosine kinase domain of both EGFR and HER2-neu genes were amplified by two rounds of polymerase- chain-reaction assays with the use of Taq polymerase (Applied Biosystems).
C-20/A4 cultures were analyzed for apoptotic cell death by Annexin V and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays with the use of cytoplasmic lactate dehydrogenase (LDH) release as a measure of necrosis.
We measured total folate of the plasma samples by microbial assay with the use of Lactobacillus casei [ 17].
Folate concentrations in colorectal biopsies were measured by standard microbiological assay with the use of Lactobacillus rhamnosus (ATCC 7469) (33).
However, the loss of responses and therefore the loss of sensitivity of an assay with the use of cryopreserved samples instead of fresh must be minimized.
At this point, we looked to combine the advantages of a screening assay with the use of our in vivo spliceosensor approach.
We measured total folate of the plasma samples by microbial assay with the use of Lactobacillus casei, as we have described previously (14, 15).
IGF-binding proteins-1 was measured by a coated-tube immunoradiometric assay with the use of the DSL-10-7800 Active kit (Diagnostic System Laboratories).
Total cholesterol, high-density lipoprotein cholesterol and triglyceride levels were analysed by colorimetric enzymatic assays with the use of an autoanalyzer HITACHI-71700, HiTokyo, Japan, Japan).
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