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Peak viraemia levels for each species are shown as relative plaque-forming units (pfu; bars represent means), estimated by extrapolation from a standard curve generated using serial dilutions of RNA isolated from the challenge virus and assayed using the same method as the test samples26 (b, sheep; e, goats; h, cattle).
The reaction mixtures assayed using the same procedure in the absence of MamK proteins served as negative controls.
The extracted supernatant was assayed using the same bile acids kit as above after dilution of 1∶5 with PBS.
Serum creatinine was assayed using the same method as described above for urinary creatinine.
Data is presented as percentage of total DNA assayed using the same primer pairs at each site.
HTLV-1 tax mRNA expression was assayed using the same method, except that cDNA from the Tax+ cell line MT2 was used to generate the standard curve.
Similar(51)
FLPe assays used the same conditions as Cre assays except that the annealing temperature was raised to 70°C.
For example, such intuitive knowledge would have predicted small differences in performance between Assay 1 and Assay 2 because both assays used the same primers at the same A&E temperature.
In this study, the newly developed triplex real-time PCR assay was compared with a quadruplex conventional gel-based PCR assay using the same primer sets in both assays.
The multiplex RT-nPCR and real-time RT-PCR for detecting BVDV-1 and -2 were developed by Gilbert et al. (1999) and Aebischer et al. (2014b) respectively (Table 1), and these were used to compare with the PNA-AuNP colorimetric detection assay using the same templates on sensitivity.
The reproducibility of the two DArT genotyping arrays was examined by independent assay using the same DNA.
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