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We also assayed the interaction of Atf7ip with two of the known major mediators of Xi-maintenance, Xist expression and DNA methylation.
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To enhance the conditioning regimen's antileukemic effect, we decided to supplant Flu with clofarabine (Clo), and assayed the interactions of these nucleoside analogs alone and in combination with Bu in Bu-resistant human cell lines in vitro.
In order to interrogate whether this motif was responsible for this interaction, we constructed additional yeast two-hybrid vectors containing the domain of PFC4 and PFC16 alone in each bait/prey configuration and assayed the interactions of these with each of the full length open reading frames.
For example, global interactome studies with C. elegans proteins assayed the interactions of 25 of the nematode's 62 PDZ domain proteins.
Moreover, based on Y2H assays, the interaction of CLIPR-59 with RIP1 but not Caspase-8 or FADD, appears to be direct.
Competition and antibody supershift assays confirmed the interaction of DNA protein complex.
This allowed further assays for the interaction of CRASP-3 and CRASP-5 with the CFHRs serum proteins.
To identify the mechanism of BRMS1 nuclear transport, we analyzed by a yeast two-hybrid assay the interaction of BRMS1 protein with several importins, belonging to different subfamilies based on the similarity of their primary structure [23].
However, as expected from the GST pull-down assay, the interaction of CaM12 with the N-terminal CaMBD of AC8 appears to be even stronger.
Although the unavailability of suitable antibodies and technical challenges prevented us from confirming the direct binding of FN-fs to chondrocyte TLR-2 in the immunoprecipitation assay, the interaction of fluorescently labeled 29-kDa FN-f with chondrocytes was abolished by using excess TLR-2 antibody, suggesting a direct interaction (data not shown).
(A) Pull-down assay for the interaction of SCARB2 or deglycosylated (DG) SCARB2 with EV71 mature virions in vitro.
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