In long-lived protein degradation assays, the contribution of macroautophagy to the total autophagy can be approximated as the percentage of protein degradation inhibitable by lysosome inhibitors that is also inhibitable by the macroautophagy inhibitor 3-MA [ 29].
Turnover appears to be rapid, yet none of these studies examined Gadd45a expression constructs co-transfected with hemi-methylated reporters, in order to assay the contribution of endogenous remethylation and silencing to turnover rates after methylcytosine is removed from one strand of a 5MeCG/G5MeC sequence.
Next, we assayed the domain contribution of SR proteins in mediating β-catenin accumulation.
After assaying separately the contribution of CG dinucleotide frequency and the effect of TF binding, we tested the relative ability of each parameter to explain methylation patterns genome-wide.
Additionally, we assayed the relative contributions of an adverse extra-uterine compared to an adverse intra-uterine environment in the development of this phenotype by cross-fostering a subset of offspring to dams on the opposite diet during the suckling period.
To assay the potential contribution of PTEN loss in breast tumor promotion, Li et al. [ 1] crossed Pten heterozygous mice with mouse mammary tumor virus- Wnt-1 transgenic (Wnt-1 TG, Pten+/-) mice.
To assay the specific contribution of GvP1 cross-linking and other wall matrix proteins to the reduction in hydration, GvP1 levels in cell walls were manipulated in vitro by binding selected fractions of extracellular proteins and their effect on wall hydration during H2O2 incubation assayed.
A neutralization inhibition assay revealed the contribution of the V3 antibodies to the neutralizing capacity of the ITM1 plasma.
The assay for the contribution of the βα-hairpin clamps to the stability of three TIM barrel proteins involves the replacement of the polar side chain H-bond acceptors, asparagine, aspartic acid, glutamic acid and serine, with alanine.
