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In summary, the ASE pipeline assayed in two contrasting conditions yielded a list of promising genes for further study.
In a paired format, where sera were assayed in two separate ELISAs using ORF4 peptides derived from the genetically very closely related Porcilis and Lelystad PRRSV strains, it was possible to differentiate between antibodies induced by these two viruses.
The PHS cases were assayed in two analytic runs [23], [24].
A case control analysis was performed using 958 single nucleotide polymorphisms (SNPs) spanning the MHC assayed in two independent datasets.
Every serum sample was assayed in two independent HI tests against the formaldehyde inactivated 2009 A/H1N1 influenza virus (X-179A reassortant strain) and the seasonal H1N1 influenza virus (IVR-148 reassortant strain).
The glutaminase activity of PfPdx2 was assayed in two steps, according to [5], [6], by measuring the formation of glutamate, which is subsequently converted to 2-oxoglutarate by glutamate dehydrogenase with acetylpyridine adenine dinucleotide (APAD) as co-substrate.
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All test compounds were assayed in four replicate wells (n = 4).
Additionally, the applicability of this method has been successfully assayed in ten commercial vegetable oils and derivatives.
The solar radiation has been assayed in four wavelength bands (UV-C, 170 280 nm, UV-B, 280 315 nm), UV-A (315 400 nm) and PAR (photosynthetic active radiation, 400 700 nm).
Each dilution was assayed in three replicates.
Each sample was assayed in six wells on the 96 well plate, three containing ouabain.
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