Sentence examples for assayed in extracts of from inspiring English sources

Flow cytometric analysis of apoptosis in Jurkat cells are given in supplementary Figure 3. Caspase-3 activity was assayed in extracts of control Jurkats and Jurkats cocultured under a variety of conditions with tumour cells.

To test this hypothesis we assayed AGO2 in extracts of non infected and TCV-infected ago1-25 mutand and wild type Arabidopsis by western blotting.

To verify that brain glucose levels were also elevated in our STZ rats, the glucose concentration was assayed in ethanol extracts of cerebral cortex dissected from funnel-frozen brains of two STZ rats, using previously described methods (Dienel et al., 2007).

Autoantibodies as well as total IgM and IgG were quantified by enzyme-linked immunosorbent assay in extracts of synovial tissues and matched serum from patients with RA or osteoarthritis (OA).

CT-L proteasome activity was assayed in crude extracts with the hydrolysis of fluorogenic peptide LLVY-AMC at 37°C for 30 min, as described previously [32].

Conventional assays of ATase expression in extracts of biopsy material are extremely sensitive but produce tissue-average values and take no account of cellular heterogeneity (Lee et al, 1991; Watson and Margison, 2000).

Plasma and retina extract samples were assayed in triplicate of 50 μl/well in a 96-well black/clear bottom plate (BD Biosciences, Franklin Lakes, NJ) for fluorescence with a SpectraMax Gemini EM fluorescent plate reader (Molecular Devices, Sunnyvale, CA) based on standard curves of FITC-BSA with excitation at 488 nm and emission at 520 nm.

Each extract was assayed in 50 μl of reaction mixture containing 20 mM Tris-HCl (pH 8.3), 1.5 mM MgCl2, 63 mM KCl, 0.05% Tween 20, 1 mM EGTA, 50 μM dNTPs, 0.1 μg of 5'-DIG end-labeled TS oligonucleotide (5'-AATCCGTCGAGCAGAGTT-3') and 2 units of Taq DNA polymerase (Boehringer Mannheim).

The purpose of the study is to develop a suitable analytical method in order to establish appropriate conditions for isolation and assay of the dominant compounds in extracts of Capsicum annuum.

To our knowledge this represents the first direct assay of GDP- L-galactose hydrolysis in extracts of plant tissues.

LDH activity was spectrophotometrically assayed in the cytosolic extract or in the of cell cultures by adding aliquots to 1 ml reaction buffer (50 mM Na2HPO4, pH 7.0, 0.15 mM NADH, and 0.3 mM pyruvate).