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Moreover, there was a delay in endoderm differentiation as assayed by staining with an antibody that recognizes Endo1.
AP expression was assayed by staining.
Survival of proliferating cells was assayed by staining for BrdU.
The extent of biofilm formation was assayed by staining with crystal violet.
Cell cycle profile was assayed by staining with or propidium iodide (PI) or BrdU incorporation as described before.
Young panicles of PA64S of the two phenotypes at the meiosis stage were collected, and the pollen fertility was assayed by staining with potassium iodide (1% I2-KI).
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This activity was assayed by MTT staining as described by Mosmann [ 25] and adapted by Stábeli et al. [ 21].
Importantly, Rab2A and its Q58H mutant colocalized with Erk1/2 at the ERGIC, as assayed by ERGIC53 staining.
At the end of the induction period, neurogenic differentiation was assayed by immunofluorescence staining for neural and glial-specific protein expression.
Cell death was assayed by DAPI staining cells after exposure to indomethacin, and scoring nuclei for condensed chromatin and fragmented nuclei.
Apoptosis was assayed by TUNEL staining using the in situ TUNEL cell death detection kit according to the manufacturer's instructions.
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