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Umbilical cord plasma interleukin-6 concentrations were assayed by a sensitive and specific immunoassay.
SOD activity assayed by a xanthine xanthine oxidase method was higher in EDAs compared with EDVs (P<0.05).
This biosensor consists of an allosteric fusion protein, which is expressed using cell-free protein synthesis technology and is directly assayed by a colorimetric response.
Proliferation was assayed by a Coulter Counter, while cholesterol biosynthesis was measured by the incorporation of 14C-acetate into cholesterol, under the same experimental conditions.
The migration ability of RH30 cells stably overexpressing TBX3 was assayed by a scratch wound assay and we found that TBX3 also inhibited cell migration as assayed by wound recovery (Fig. 3d).
This was assayed by a standard method [17].
PG was assayed by a modified ruthenium red assay (Torres et al. 2011; Ortiz et al. 2014).
Total GPX activity was assayed by a method using tert-butyl hydroperoxide and reduced GSH as substrates [47].
The concentration of the samples was assayed by a HPLC system (Waters-Alliance 2690, USA) at 210 nm (USP 34).
As assayed by a combination of fresh weight and chlorophyll concentration, 2-ethylhexanal had a negative impact on two-week-old vegetative plants.
Cell growth rates were assayed by a nonradioactive cell proliferation assay kit (Promega).
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