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In the multiple dose phase AChE was assayed at the time of drug administration on days 1, 3, 5, 8, 10 and 12 (drug days).
A day 0 plate was set up to define the baseline cell number; this plate was assayed at the time of treatment.
Cell death and apoptosis were assayed at the time points indicated in the figure legends after treatment with cell death stimuli.
Plasma samples from all studies were assayed at the time of each study in the Analysis Center (Sumika Chemical Analysis Service, Ltd., Osaka, Japan).
Apoptosis was assayed at the time points indicated in the legends to Figs. 5, 6, and 8. Substrate absorption indicative of apoptosis was measured at 405 nm (reference 490 nm).
Serum samples were obtained after an overnight fast at entry into the DCCT study (between 1983 and 1989) and assayed at the time for hemoglobin A1c (HbA1creatininenine, and lipids, and aliquots were stored at −70°C.
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41 However, this was largely based on the sensitivity of available laboratory assays at the time.
Paired bilirubin assays at the time of CA19.9 measurement were available for all patients included in the analysis.
For CFU-F assays, at the time of euthanasia, bone marrow from femoral, tibial and humeral medullary cavities were collected, and cell numbers were determined after removal of red blood cells with Zapoglobin (Coulter Corp., Miami, FL, USA).
DENV plasma viremia levels were measured using an internally controlled, serotype-specific, real-time reverse-transcription polymerase chain reaction (RT-PCR) assay, with serial samples from each patient assayed at the same time [ 35].
Parallel cohorts were assayed at the same time under the same conditions and the experimenter was blinded to the genotypes or experimental conditions.
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corroborated at the time
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estimated at the time
investigated at the time
ascertained at the time
quantified at the time
measured at the time
sampled at the time
evaluated at the time
overestimated at the time
scrutinised at the time
analysed at the time
assayed at the isoform
assayed at the test
assayed at the beginning
assayed at the level
assayed at the transcript
assayed at the completion
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