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Exact(10)
Fig. 3 Cell viability of the nanomachine by CCK-8 assay with different concentrations of extract solutions.
No matrix effects were observed on the performance of the assay with different species (mussels, scallops, oysters, clams, and cockles).
The cytotoxicity of EILDV-conjugated and non-conjugated micellar formulations were carried out on B16F10 cell line by the MTT assay with different drug concentrations and incubation times.
Due to poor solubility of CoQ10 in aqueous solutions, we repeated the assay with different formulations of CoQ10 in accordance to its lipophilic requirements.
The specificity of the interaction was further confirmed by GST pull-down assay with different amount of bait protein.
The enzyme assay with different aliquots of enzyme (0.02 mL–0.5 mL) is depicted in Figure 7(b).
Similar(50)
Splenocyte proliferation in vitro was assayed with different concentrations of EPSAH.
Biotransformation assays with different substrates reveal a successful immobilization of the d-Carb with similar specific activities, whereat the substrate NCPheAla was converted with the highest specific activity of 12.91 U/mg.
When submitted to assays with different substrates and inhibitors, it showed selectivity for succinyl-l-ala-ala-pro-l-phenylalanine-p-nitroanilide and was almost completely inhibited by phenylmethylsulfonyl fluoride, behaving as a chymotrypsin-like protease.
Cord blood volume and nucleated cell content were measured, and the number of hematopoietic progenitor-stem cells was determined by means of fluorescence-activated cell sorting with the CD34+ epitope and by means of colony assays with different hematopoietic growth factors.
PBMC-based assays with different readouts were compared.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com