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Cytotoxicity and anticancer efficiency were evaluated using MTT assay, where a redox reaction occurs between the MTT in DMSO and intracellular succinate dehydrogenase.
An enhanced angiogenic effect was also seen in vivo using the chick embryo chorioallantoic membrane assay, where a significant increase in the number of blood vessels converging towards collagen spheres was observed compared with control PCL spheres.
To monitor the purification process we developed a novel HPLC activity assay where a sequential order of phosphorylation was observed whereby the first tyrosine residue was completely phosphorylated prior to phosphorylation of the tandem tyrosine residue.
This is an assay where a DEVD peptide substrate is cleaved by active caspases to release aminoluciferin as a substrate for luciferase to produce light.
It was determined using the incubated tubes from the macrodilution assay, where a 100 μl sample from each tube was inoculated into petri dishes containing nutrient agar.
(A ) Competition assay where a sample containing SCΔ60 and 3.0 equivalents of CpxI(47-134) was titrated with WT CpxI(26-83).
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In the past, endotoxin was measured in the blood using a limulus assay where an aqueous extract of blood cells (amebocytes) from the horseshoe crab (Limulus polyphemus) is combined with a pyrochrome yielding a chromogenic readout.
The mitochondrial DNA content was quantified by utilizing a fluorometric assay where an increase in SYBr Green (Invitrogen Molecular Probes) fluorescence because of higher DNA binding was quantified as a measure of the amount of DNA content.
One such system could be a retrotransposition assay, where an L1 reporter construct has been designed so that translation of the reporter (drug-resistance gene or enhanced green fluorescent protein) occurs only after L1 reverse transcription and insertion of its cDNA copy into the genome [ 88, 89].
With that said, there were a subset of biological assays where a clear separation between the NN and NI pairs were found.
With that said, there were a subset of biological assays where a clear separation between the NN and NI pairs found.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com