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The results from the DPPH radical scavenging assay were validated by 2,2′-azinobis 3-ethylbenzothiazoline-6-sulfonate 2,2′-azinobis 3-ethylbenzothiazoline-6-sulfonate 2,2′-azinobis 3-ethylbenzothiazoline-6-sulfonate 2,2′-azinobis 3-ethylbenzothiazoline-6-sulfonate 2,2′-azinobis 3-ethylbenzothiazoline-6-sulfonate
Briefly, the results of each assay were validated by determination of EC50 values produced by the standard antimalarial drugs against CQ resistant P. falciparum.
DNA methylation values obtained from the GoldenGate methylation assay were validated by pyrosequencing in those samples used for the discovery phase (PXA 4, 5), as well as on an independent set of validation samples (PXA 6-11).
Significant CpGs from the second data set that were not included in the 27K assay were validated by pyrosequencing in a subset of 476 samples from the first data set.
Therefore, cDNA prepared from RNA extracted with protocol 1 might had slightly more amplifiable templates per sample volume than cDNA prepared from total RNA extracted with protocol 2. The results from the multiplex endpoint RT-PCR assay were validated by the results obtained from the RT-qPCR assay and allowed the identification of FFPE samples with an adequate RNA quality.
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The assay was validated by comparison with other established CCR5 assays.
The assay was validated by using oseltamivir carboxylate as a reference inhibitor.
In addition, this assay was validated by testing clinical samples and isolates.
The assay was validated by testing serial dilutions of the WHO international HBV DNA standard.
The assay was validated by calculating specificity, sensitivity, detection limit, precision, PCR efficiency, DNA extraction efficiency and food matrix inhibition.
The specificity of the RT-LAMP assay was validated by amplifying eight strains of PPRV isolated in different geographical areas.
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