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First, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay were performed using a commercially available kit (Chemicon, Internat. Inc).
To evaluate whether NSC697923 also inhibits colony-formation ability of NB cell lines, clonogenic assay and soft agar assay were performed using a panel of six NB cell lines.
To assess the effect of FC-98 on T cells transmigration to BMDCs, Chemotaxis assay were performed using a 24 well Transwell system with 5 μm pore polycarbonate filter (Corning, NY, USA).
Similar(57)
The ROS generation assay was performed using a DCFH-DA and followed the manufacturer's instructions.
Pull-down assay was performed using a His-RIF2 protein with GST (control) or GST-RF2.
The assay was performed using a Lumipulse ƒ system (Fujirebio Inc ., which is a fully automated CLEIA analyzer.
The virus binding assay was performed using a previously reported protocol, with modifications (Qing et al., 2016).
The pyrosequencing assay was performed using a PyroGold SQA sample preparation kit (Qiagen, Hilden, Germany) according to manufacturer's instructions.
The total phenolic content assay was performed using a modified form of the procedure described by Singleton and Rossi (1965).
The IN enzymatic activity assay was performed using a previously described assay system [57], [58].
ChIP assay was performed using a chromatin immunoprecipitation assay kit (Upstates).
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