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MTT assay were done in triplicate.
The tests for validation of the assay were done using a commercial CA and the values obtained were 130.7 ± 20.1 EU mg-1 using bicarbonate as a substrate and 168.8 ± 5.9 EU mg-1 using CO2 as a substrate.
Blood was processed within one hour after sampling and phagocytosis assay were done as previously described [19].
In addition, real-time RT-PCR, western blotting, human IL-6 ELISA assay were done for the results validation.
The hypopharyngeal glands score and the Bradford assay were done by a blind test to ensure impartiality in the results.
The blastomere adhesion assay on C-cadherin substrates and the cell sorting assays, including cell reaggregation assay and cell dispersal assay, were done as previously described [1], [9].
Similar(45)
The antimicrobial assay was done using the disc diffusion method.
Hemolytic assay was done following standard protocol [22].
The preliminary antimicrobial activity assay was done by disc diffusion assay techniques.
The flow cytometry-based competitive binding assay was done by B.Z.
The DPPH assay was done according to the method previously describe with slight modifications [20].
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