Exact(6)
Band pixel intensities for GST-KIX assay were determined using the density function of Quantity One software (Biorad).
Statistically significant differences in each assay were determined using spss version 18.0 (SPSS, Chicago, IL, USA).
The sensitivity, specificity, and positive and negative predictive values of the TBcID assay were determined using the results of the Accuprobe MTBC culture identification test as the gold standard.
IC50 values, which represent the concentration of the extract required to scavenge half the ABTS+ radical, for the antioxidant assay were determined using GraphPad Prism Version 4.00 for windows.
Migration assay and invasion assay were determined using a transwell system (Corning Costar; Acton, MA) with an 8-μm pore size membrane coated with fibronectin for migration assay or with matrigel for invasion assay as described by Li et al. [ 17].
The detection limit and the amplification efficiency of the D. nodosus-specific real-time PCR assay were determined using 10-fold serial dilutions of chromosomal DNA (393 ng to 3.9 ag corresponding to approximately 3 × 108 to 3 × 10-3 cofies of the D. nodosus genome per reaction) obtained from D. nodosus CCUG 27824T (CCUG), and performing real-time PCR as described above.
Similar(54)
The sensitivity of the assay was determined using quantified rotavirus stocks and a plasmid DNA stock.
Reporter assay was determined using a dual luciferase assay kit (Promega) as previously described [45].
The quality and reproducibility of the assay was determined using the quality controls provided with the kit.
MTT reduction assay was determined using the in vitro toxicology assay kit.
The C2P® assay is determined using a combination of the specific activity of CDK1 (CDK1Sandand CDK2 (CDK2SA).
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