Sentence examples for assay were created by from inspiring English sources
On the other hand, it is possible that coding sequence and 5'-UTR interactions identified in our assay were "created" by association with Ago2 post-lysis.
The pFSVpur-TC-I4-PC construct to be used as a positive control during the PCR screening assay was created by PCR amplification with Q5 Hot Start High-Fidelity DNA polymerase (NEB) using primers 5′-CTCTCTGGAGGGCTAGCTGTAAAACGACGGCCAGT-3′ and 5′-CTGGGACTACACTGGCGTTACCCAACTTAATC-3′ followed by digestion with DpnI (NEB) and blunt ligation with T4 Ligase (NEB).
The UAS-Arctic Aβ42, UAS-Aβ42 and UAS-Aβ40 transgenic flies that were used for all survival assays were created by site-directed insertion at the 51D location on the second chromosome using the φC31 integrase system as previously described (Bischof et al., 2007; Kruppa et al., 2013).
Luciferase assays: Luciferase assay constructs were created by ligating PCR products into pGL4-11 (Promega) upstream of the luciferase gene.
For autophagy induction alkaline phosphatase assays, pho13 ∆ pho8 ∆60 strains were created by crossing a pho13 Δ:: URA3 pho8 ∆60:: HIstrainain (kindly provided by Daniel Klionsky) to wild-type and tdna mutants.
Three dimensional (3D) culture conditions were created by using the Matrigel "on-top" assay [ 36].
To investigate the potential antibacterial properties an in vitro adhesion assay was created.
Multiplex assays can be created by mixing bead sets with different conjugated capture antibodies to simultaneously test for many (up to 50 or more) analytes in a single clinical sample (Elshal and McCoy, 2006).
In this assay, an artificial wound is created by scratching a confluent monolayer of cells and the ability of the cells to move into and close the wound is thought to predict their migration ability in vivo (Liang et al, 2007).
