Exact(1)
The synthesis of cDNA and the RT-qPCR assay were completed by the Advanced Analysis Centre's Genomic Facility at the University of Guelph.
Similar(59)
aImmunofluorescence assay was completed by Western blot and cross-adsorption as described in the text.
All PRNT assays were completed by the same technician using the same standard assay procedure.
Assays were completed by a sole technician who was blinded to the case control status of the samples in the laboratory of Dr Lau at the University of Calgary.
Microtitre growth assays were completed by inoculating 150 μl of MM (Difco yeast nitrogen base without amino acids, 0.002% adenine, 0.002% uracil, 0.002% L-histidine, 0.01% L-leucine, and 2% glucose) + Phe Tyr (where Phe was kept constant at 0.003 mM and Try was varied from 0.003 mM to 1.2 mM) in a 96 well microtitre plate.
All WNV screens and titrations for virus quantification were completed by plaque assay on Vero cell culture [ 73].
The hybridization assay was completed in 50 min.
The assay was completed within 2 h from DNA extraction.
This assay was completed in 2 days.
Each assay was completed in triplicate.
The assay is completed in 6 minutes.
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