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The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the assay were calculated for each category.
Each tube was analyzed five times, where the number of flies that passed the line was counted, and the median values of 25 (5×5) locomotor assays of five flies per assay were calculated for each group.
PCR detects the causative microbe in 35/47 cases of culture-proven IE and provides an aetiological agent in 43/69 cases of culture-negative IE. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the 16S rDNA PCR assay were calculated for this series of selected samples using the clinical diagnosis of IE as the reference standard.
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The inhibition concentration (IC50) value for the DPPH scavenging assay was calculated for all the four solvent extracts of coffee pulps.
Patients were characterized using simple descriptive statistics, and the sensitivity of the Xpert assay was calculated for patients stratified by CD4 cell counts and sputum smear status.
r = N ΣXY - ΣX ΣY NΣ X 2 - ΣX 2 NΣ Y 2 - ΣY 2 Distances between samples and assays were calculated for hierarchical clustering based on the ΔCT values using Pearson's correlation or the Eucidian distance calculated as follows [ https://products.appliedbiosystems.com].
The IC50 values for the tyrosinase assay were calculated from the logarithmic non-linear regression curve derived from the plotted data using GraphPad Prism software version 4.03 (GraphPad Software, Inc., San Diego, USA).
The units for this assay were calculated by dividing the measured A420nm (released nitrophenol) by the time allowed for the reaction and volume of permeabilized cells used for the reaction.
PCR efficiencies for each assay were calculated based on a separate experiment with a serial dilution of pure ERCC cDNA (in the absence of background RNA).
Estimates of repeatability standard deviation (SDr) and relative standard deviation (RSDr) for each assay were calculated as suggested by Horwitz [ 32].
The cut-off values for the assay were calculated as the mean specific optical density (OD) plus three standard deviations (SDs) using serum samples of non-immunized mice (Day 0) assayed at a 1 100 dilution.
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