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The specificity and sensitivity of the PCR assay were assessed using genomic DNA from clonal cultures, plasmid copy number of cloned target sequences, as well as from sediment samples.
The results of the vancomycin assay were assessed using the rsm package version 2.0 (IBM, Armonk, United States) and R version 2.15.3 (Bell laboratories, Murray Hill, New Jersey).
Relative DNA methylation levels of the SOCS2 gene locus referred to as total DNA as determined with a methylation-unspecific β-actin (ACTB) assay were assessed using a quantitative duplex PCR.
To determine whether the exclusion of nonviable cells, determined with the LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (L34957; Life Technologies, Carlsbad, CA, USA), would change the staining distribution of cells, selected patient samples for this assay were assessed using the FACSAria III system.
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The assay was assessed using a full panel of BTV reference strains and clinical samples.
The assay was assessed using a list of 137 well-defined intestinal bacterial strains or isolates.
The in vitro tube formation assay was assessed using ibidi μ-Slides (15-well, ibidi GmbH, Martinsried, Germany) in accordance with manufacturer's protocol.
The analytical specificity of the assay was assessed using blood samples positive for Hepatozoon canis, Ehrlichia canis, Anaplasma platys, Babesia microti, Babesia rossi and Theileria annae (syn. Babesia vulpes).
The variation in the performance of the assay was assessed using artemisinin.
The reproducibility of the assay was assessed using one ng DNA of LVS tested in three replicate runs.
The limit of detection of the Taqman assay was assessed using a dilution series of DNA from isolates of B. pseudomallei and B. mallei.
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