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The multiplex assay was verified testing genomic DNA of 24 carbapenemase-producing strains.
High specificity of the assay was verified with seven other bacterial strains.
Sensitivity and specificity of the assay was verified using a panel of arboviruses.
Precision of the assay was verified by determination of the inter- and intra-assay coefficients of variation, which were <15 and <10 %, respectively.
The specificity of this assay was verified through identifying unrelated (sheep, horse, deer, donkey, rabbit, goose, goat, shrimp, salmon and maize) mitochondrial DNA as species-specific targets.
The assay was verified in sample food matrices by spiking white grape and peach mango juices as well as apple cider.
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Results of the H11 microneutralization assay were verified by horse erythrocyte HI assay that used subtype H11N9 virus.
In additional experiments, the accuracy and efficacy of the quantitative RT-PCR assay were verified by employing competitive PCR.
The amount of recombinant HSP70 and OLA1 proteins used in this assay were verified by Coomassie blue (Invitrogen) staining.
To evaluate the achieved differences of drug susceptibility of the TGCT cells in normoxia and hypoxia, the results from the colorimetric MTT assay were verified by flow cytometry.
Moreover, the performance characteristics of the assay were verified in an independent molecular pathological laboratory to confirm that the test meets its specifications when used in a routine diagnostic laboratory.
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