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The assay was validated using calcium phosphate cement and PMMA as biomaterials.
The PCR assay was validated using DNA from selected pure cultures.
The LAMP assay was validated using 28 T. foetus and 35 non-T.
The mPCR assay was validated using naturally infected field samples for one or more citrus viruses and the huanglongbing bacterium.
The assay was validated using human serum ED50 (dilution of serum effecting 50% neutralization) as the primary reportable value (RV).
Finally, the assay was validated using DNA extracted from clinical respiratory specimens for WU and KI polyomaviruses and the results were confirmed by direct nucleotide sequencing.
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19 Key elements of this assay were validated using fresh-frozen brain slices taken from AD brains.
Each of the antibody-capture assays was validated using a large number of serum samples (n >1100) based upon the assay validation methods of Jacobson, which is supported by the Office International des Epizooties [ 25].
The assays were validated using engineered plasmids and/or genomic DNA samples that are either homozygous wild type or heterozygous for one of three SNPs that lead to the RyR1 amino acid substitutions T4826I, H4833Y, and/or R4861H.
Quantitative assays were validated using SYBR Green chemistry.
All assays were validated using the cellular controls GAPDH (transcriptionally active) and rabbit centromere (transcriptionally silent), and only samples with >2-fold enrichment of H3K4me2 for GADPH were used in our final analyses.
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