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Untreated cells served as controls; the assay was terminated at 72 h, and cell viability was determined via colorimetric determination.
After incubation at 37°C for 30 min, the assay was terminated upon the addition of 1 μL of 100% trichloroacetic acid (TCA).
The assay was terminated by the addition of 15 µl of 2×SDS sample buffer and boiling of the samples for 5 min. The sample was then subjected to SDS-PAGE and exposed to photographic film for autoradiography.
The cells were treated with 1× DCFH-DA solution in DMEM for 30 min at 37°C, washed with PBS, and the assay was terminated by the addition of 2× Cell Lysis Buffer.
The assay was terminated by the addition of cold TCA.
The assay was terminated after a further 7-day incubation.
Similar(37)
Assays were terminated and results discarded if control mortality exceeds 10% at any time.
Assays were terminated with guanidine hydrochloride.
The assays were terminated by washing cells with ice-cold PBS.
Assays were terminated at 10 min by the addition of 15 µl 50% HCL, 200 µl 12.5% sodium nitrite and 200 µl 12.5%.
Assays were terminated by the addition of 6% (wt/vol) SDS.
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