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Plates were sealed in plastic bags and stored at 4°C until the assay was started.
The XTT assay was started 24 h after the medium change when new medium supplemented with nanoparticles was added.
The assay was started by the addition of supernatant of 293FT cells and the lysozyme absorbance was recorded every 30 s over 20 min using a spectrophotometer.
The nitrite assay was started by adding the sample (360 µg proteins in 50 µl) to 50 µl sulfanilamide solutions in 96-well plates in duplicate.
The assay was started by adding 60 µM decylubiqunol and absorbance recorded for one minute, taking the initial 30 seconds rate for calculation.
Cultures were then incubated for 5 d in vitro (DIV) at 37°C and 7% CO2 before the neurotoxicity assay was started.
Similar(33)
The assay is started by mixing DUB and diUb solutions, and 5 μl aliquots are taken at indicated time points.
This could look like an apparent inhibition of the complex I-dependent reaction if the assay is started with a partially de-activated enzyme.
The assays were started with 0.15 mM deamino-NADH.
The oxidase assays were started with 0.25 mM deamino-NADH (extinction coefficient 6.22 mM−1 cm−1) and the absorbance monitored at 340 nm for 2 minutes.
All assays were started by addition of substrate.
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CEO of Professional Science Editing for Scientists @ prosciediting.com