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The SPR assay was run in the running buffer (20 mmol/L Tris-HCl, pH 7.5, 200 mmol/L NaCl and 0.005% Tween 20) at the flow rate of 30 μL/min.
The assay was run in triplicate.
Another reason for the loss of activity can be related to enzyme denaturation in each time the assay was run 15.
Six RT-LAMP primers were designed from the same conserved region of RT-nPCR, and the assay was run at 63 °C for one hour.
Following exposure the MTT assay was run as previously described.
The assay was run in triplicate for each sample.
Each TaqMan assay was run in four replicates for each RNA sample.
A conventional genotyping assay was run in parallel, based on nested PCR amplification (Figure 6C).
To confirm successful DNA extraction a human C reactive protein real time assay was run for each DNA extraction.
If plasma was not sent, a qualitative NAAT for HIV-1 RNA (Gen-Probe Procleix HIV-1 RNA Assay) was run off-label on remnant sera.
For every two trials a control assay was run, in which the acetone-treated net was used in place of the insecticide treated one.
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