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The assay was controlled using Escherichia coli ATCC 25922.
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Samples of patients with different diagnoses and control participants were analysed together in the ELISA templates, and possible variability between different assays was controlled using 4 samples of known BDNF concentration.
The quality of the assays was controlled by using P. falciparum reference lines (3D7 Africa, CQ-sensitive clone and FCM29 Cameroon, CQ-resistant clone).
The assay was validated using control human plasma spiked with known amounts of DMXAA, and found to be linear from 50 n M to 2 m M. Intra- and interassay recovery was between 102 and 104% and CV ranged from 0.2% at 20 μ M to 1.9% at 500 μ M. Free plasma DMXAA concentrations were determined by ultrafiltration.
The quality and reproducibility of the assay was determined using the quality controls provided with the kit.
The specificity of the GeXP assay was examined using positive controls for each virus.
(D) The wound-healing assay was performed using the control NIH3T3 cells and NIH3T3 cells expressing Abi-1 or NESH, and analyzed as described in Fig. 1e.
The repeatability of the genotyping assay was evaluated using 14 positive controls.
Soft agar colony formation assay was performed using transfected and control cells.
The accuracy of the assay was assessed using reference quality-control urine specimens supplied by Centres for Disease Control and Prevention CDCC), Atlanta, Georgia, USA; the detection limit was <5.0 μg/L.
Negative control ChIP assay was performed using antibody to glyceraldehyde 3-phosphate dehydrogenase (anti-GAPDH) (Abcam) or secondary Ab (Dako) only.
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