The phrase "assay was calculated using" is correct and usable in written English.
It can be used in scientific or technical contexts when describing the method or process by which an assay's results were determined.
Example: "The assay was calculated using a standard curve derived from known concentrations of the analyte."
Alternatives: "assay was determined by" or "assay was derived from".
The relative inhibition rate of the circle mycelium compared with the blank assay was calculated using the following equation.
The corrected fluorescence of the encoded microbeads before and during sandwich assay was calculated using ImageJ by the following formula: {text{Corrected}};{text{total}};{text{fluorescence}}, =,{text{Integrated}};{text{density}},, ( {text{Area}};{text{of}};{text{selected}};{text{beads}}, times,{text{Mean}};{text{fluorescence}};{text{of}};{text{background}};{text{readings)}} (1).
The sensitivity and specificity of the ALS assay was calculated using non-TB patients as controls.
From the concentration of DNA, the number of copies per assay was calculated using the 1C value (haploid genome mass in pg) derived from several references.
The statistical significance of protein enrichment in c-ponatinib assay versus free ponatinib competition assay was calculated using the modification of the Decontaminator method.
Compound reproducibility within each assay was calculated using the 66 replicate compounds in the NTP library (Huang et al. 2011), leaving 1,340 unique compounds.
Sensitivity and specificity of the significant lactate assay were calculated using a cutoff 1.5 times the upper normal range of lactate (2.2 mmol/l) in the two-by-two tables.
Initial angles for the pipette assay were calculated using the pipette location as a reference.
The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of each qRT-PCR assay were calculated using standard procedures.
Statistics for both the melanosome transport and vision startle assay were calculated using the one-way ANOVA paired with the Tukey honestly significant difference test.
The percentage PCR amplification efficiencies (E) for each assay were calculated, using the slope of the semi-log regression plot of cycle threshold versus log input of cDNA (10-fold dilution series of five points), with the following equation: A threshold of 10% above or below 100% efficiency was applied.
