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Hepatocyte viability was assessed in a trypan blue exclusion assay: viability was consistently >85%.
Cell viability assay Viability of RINm5F cells after treatment with the plant extracts and standard drug was determined by assaying for the reduction of 3- 4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) to formazan.
To assay viability, an aliquot of cells from a culture started at 5×10 cells/ml in 5 ml of ASM was taken every 2 days, and serial dilutions were plated with K. aerogenes bacteria onto SM/5 plates.
We confirmed the specificity of the effect observed with R59949 inhibitor in a dose response assay; viability of ISP thymocytes did not seem to be affected by DGK inhibition (not shown), contrary to the described DP sensitivity to this treatment.
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The effect of ART on cell death induction was tested in multiple assays, including viability assays and the cytotoxicity assay.
To assay the viability of B. burgdorferi, the SYBR Green I/PI assay or LIVE/DEAD BacLight bacterial viability assay was performed.
This was also noted in the MTT assay, where viability of these groups decreased but it was not statistically significant.
Invasion assay, cell viability assay to check the drug resistance and NF-κB activity assay were performed as described previously [3], [13], [19].
Triplets were used for the cytotoxicity assay, cell viability assay and RT-qPCR.
Cells were subjected to invasion assay and viability assay 48 h post-transfection, unless otherwise stated.
Cells were assayed for viability using CellTitre Glo 72 h after transfection as per the manufacturer's protocol.
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