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Indeed, our bioinformatic analysis followed by luciferase assay verification identified several genes as potential miR-9 targets.
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Mycoplasma assays, verification of morphology and growth curve analyses were performed routinely for all cell lines.
Mycoplasma test assays, verification of morphology and growth curve analysis were performed as a routine protocol for all of them.
Here, we employed SRM assay for verification of SILAC data, since we have previously validated its accuracy and effectiveness for verification of candidates in amniotic fluid [ 15].
To facilitate development of a LC-SRM assay for verification of the exploratory iTRAQ findings, we first compiled an MS/MS spectra library of dimethylated peptides from which appropriate surrogate peptides were selected.
In detail, 96 well ELISA plates (Dynex Corporation, Chantilly, VA, USA) coated with the various proteins were used in biochemical assays for verification of protein:protein interaction.
After single-assay RT qPCR verification analysis, only miR-15b showed statistically significant differences in expression levels between the two groups.
A paper describing the conceptual idea of using passive neutron assay for the verification of large size uranium samples in fuel fabrication plants was first presented at the 2001 ESARDA conference.
As a positive control for the depletion verification assay, and to enumerate cultivable microbes with the fecal collection procedure, fecal pellets from untreated mice were collected with the above described procedure.
Antigen coating was performed as described above (Enzyme linked immunosorbent assay (ELISA) for verification of immunogenic proteins).
For the scFv titration ELISA the antigen was coated as described above (Enzyme linked immunosorbent assay (ELISA) for verification of immunogenic proteins).
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