Sentence examples for assay using the full from inspiring English sources

In order to investigate whether or not the SH2 domain of Grb14 is able to bind Tie2 we performed an in vitro pull down assay using the full length Tie2 receptor and the SH2 domain of Grb14.

Promoter reporter assays using the full length fascin promoter [ 17] showed that regulation occurred, in both cases, at the transcriptional level (data not shown).

In the first assays, using the full length purified HIS-SUMO-CP1 and HIS-SUMO-CP4 after refolding, no activity was detected even after long incubation times.

The binding assays using the full-length and several truncated versions of CRE1/AHK4 (Fig. 2) confirm the hypothesis that trans-zeatin is bound by CRE1/AHK4 via the CHASE domain.

We performed this assay using the QCM™ Gelatin Invadopodia Assay according to the manufacturer's instructions.

The experimental planning was based on eight assays using the 23 full factorial design combining three important variables for PDT: photosensitizer concentrations, incubation time of cells in HY solutions and employed light dose (λ = 590 ± 10 nm).

Nuclear run-on assays and transient transfection assays using the rat hepatocyte iNOS full-length promoter and deletion constructs were designed to isolate a cis-acting regulatory element.

The activity of full-length USP11 was assayed using the fluorogenic substrate ubiquitin-AMC.

For the luciferase transactivation assays we used the full-length MMTV long terminal repeat driving transcription of the luciferase gene, pRSV-GR and the human mut or wt BRG1 containing constructs, following the previously described procedures (Johnson et al, 2008).

To test whether PPLP2 possesses EM-lytic activities, we performed an erythrocyte lysis assay, using recombinant full length PPLP2 (PPLP2RP3, see Fig.  1A).

To test this idea, we repeated the MT bridge assay using full-length yeast cytoplasmic dynein.