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Search for CNVs was performed using MLPA assay using both the P348-A2 ATprobeCACNA1A probe mix and P279 CACNA1A probemix (SALSA MLPA Kit, MRC-Holland).
Gradient time of 4 min and column temperature set at 30 °C also allow for the excellent separation of the critical peak pair (RsHNE DNPH/ONE DNPH = 3.3) within only 3.2 min. In order to reveal potential accuracy differences, a series of sample pairs were quantified based on this optimized LC MS/MS assay using both the previously used d3-DNPH and our own [N4]DNPH reagent in AIDA.
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To determine the dynamics of apoptosis induction brought about by the over-expression of the 10 pro-apoptotic proteins, all were analysed in plate-based assays, using both the TUNEL and cleaved-CASP3 assays at 12, 24, 36, 48 and 60 hours following transfection.
To assess the correlation between IMPACT multiplex assays and single automated assays, we used both the IMPACT and the Roche/Hitachi cobas c platforms to measure RF and CRP in baseline serum samples from subjects enrolled in the REFLEX study [ 10].
In this study we developed a reliable, rapid real-time PCR assay that detects a 225 bp segment of the hexon gene of both the HAdV-14p and -14a genomes on the J.B.A.I.D.S. and LightCycler 2.0 thermocycler platforms, and validated the assay using both defined preparations of isolated virus and original clinical throat swabs from recognized HAdV-14 outbreaks in military populations.
To demonstrate this we evaluated the assay using both a 96- and a 384-well plate format (Fig. 3) with B. cenocepacia strains K56-2 and K56-I2 (cepI) (Table 1).
We optimized the microbiocidal assay using both E. coli and S. aureus for four different species: coyote, house finch, garter snake, and newt.
We performed our viability assay using both methods.
Using the results of a calibration study in 295 subjects whose samples were assayed using both methods, the results of the earlier CRP assays were adjusted to the Glasgow assay, for which the current CRP international standard was used by subtracting −0.128 from the earlier log CRP assay (87.9% of the original value).
For specimens collected from 6 weeks to 18 months old infants born to HIV-1 positive mothers, assay results using both the DBS and plasma protocols agreed well with the Roche Amplicor HIV-1 DNA Test version 1.5 (95.5% agreement for DBS and 97.8% agreement for plasma).
Pre-immune serum samples collected on day 0 were tested alongside serum samples collected on day 42 in the HAI assay (see above) using both the wild-type rHA and SDV rHA as the test antigens.
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