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To detect low levels of the anthrax protective antigen (PA) exotoxin in biological fluids, we have developed a metal-enhanced fluorescence (MEF PA assay using a combination of the MEF effect and microwave-accelerated PA protein surface absorption.
The standard curve for the assay using a combination of tag-specific and ONNV-specific primers had a slope of −3.4, an R2 of 0.999, and amplification efficiency of 96.8%.
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The feed assay uses a combination of enrichment culturing and PCR to detect and enumerate viable Lactobacillus sp. HOFG1 after its application onto cattle feed.
Hemagglutination inhibition assays using a combination of these mAbs elicited a titer of 128 512 with all the tested H5N1 strains (Table 2).
Tissue from 54 histologically-identified basal cell carcinomas of the skin was obtained at surgery and assayed using a combination of functional and immunochemical procedures for matrix metalloproteinases (MMPs) with collagenolytic activity and for MMPs with gelatinolytic activity.
The C2P® assay is determined using a combination of the specific activity of CDK1 (CDK1Sandand CDK2 (CDK2SA).
The extent of population genetic divergence of the reference populations based on this assay was evaluated using a combination of traditional population genetic statistics and individual-based methods.
The cytokines were measured using capture ELISAs and the data thus obtained together with S. haematobium egg count data from urine assays were analysed using a combination of parametric and nonparametric statistical approaches.
Here, we developed a rapid and sensitive method to assay gap junction communication using a combination of single cell electrophysiology, large scale optical recordings and a digital phase sensitive detector to extract signals with a known frequency from Vf2.1.Cl, a novel fluorescent sensor of plasma membrane potential.
The entire research cohort was assayed for these 32 variations using a combination of SNPlex, SSCP analysis, TaqMan and automated DNA sequencing (3).
A non-destructive method for assaying transuranic neutron sources was developed, using a combination of γ-spectrometry and neutron correlation technique.
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