Antibacterial activity of chitosan membranes was investigated by a conductimetric assay using a Bactometer.
A CS20 colony hybridization assay using a DNA probe derived from csnA was developed.
This assay is a single-step primary binding assay using a single reagent - the QD-labeled antigenic peptides.
Compounds were screened for inhibition of HGFA activity in a kinetic enzyme assay using a chromogenic substrate.
A membrane fusion assay using a β-galactosidase reporter was adapted from Feng et. al. [69].
We next carried out an in vitro invasion assay using a Matrigel coated barrier filter.
Plasma triglycerides were quantified via a colorimetric assay using a triglyceride assay kit from Roche Diagnostics.
Liver triglycerides were quantified via a colorimetric assay using a triglyceride assay kit from Roche Diagnostics.
We assessed the properties of this HTS co-culture assay using a panel of test compounds of known activity.
The transpeptidation assay using a fluorescence resonance energy transfer (FRET) substrate was designed based on the published protocols [11],[11].
Induction of apoptosis was studied by annexin V-FITC binding assay using a kit from Beckman Coulter.
