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1,6-DCF, a sucralose hydrolysis product, is weakly mutagenic in both the Ames test and the L5178Y TK+/− mutation assay (US FDA 1998).
Sucralose has been reported to be weakly mutagenic in the mouse lymphoma mutation assay, and as noted above, its hydrolysis product 1,6-DCF was found to be weakly mutagenic in both the Ames test and the L5178Y TK+/− assay (US FDA 1998).
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Plasma total choles-terol, TG, high density lipoprotein (HDL) cholesterol, FFA, lipoprotein(a), and uric acid were assayed us-ing a Hitachi 7600 Auto Analyzer (Hit-achi InsTokyo, Japanvice, Tokyo, Japan).
The direct cosedimentation method used in our assay allows us to analyze TM-actin binding without additional TM modification.
An immunoprecipitation assay kit (US Biological, Swampscott, MA) was used to determine the tyrosine kinase activity.
Importantly, the use of a drug-tolerant assay allowed us to assess the relevance of detection of ATI in the presence of an adequate IFX trough concentration.
Thus, the high sensitivity of the nuclease assay enabled us to detect protein phosphatases despite the use of DNA substrates in the reactions.
This assay allows us to monitor each individual acetylation site on histone H3 and H4, enabling us to quantitate and compare the acetylation of all lysines simultaneously.
Caspase 3/7 assay enabled us to detect necrotic damage induced by cryopreservation.
Several disadvantages of this assay led us to design alternative tools based on flow cytometry analysis.
The resulting polychromatic and multiplexed imaging assay enabled us to measure the secretion of mycobacterial effectors inside infected host cells.
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