The two distinct tags were represented among the probes used, which was also seen as an interest to assay two detection methods.
In the assay, two kinds of graphene-polymer based nanotags were fabricated for tumor makers (TMs) distinction and signal amplification.
In this assay, two hairpin probes are designed, one of which is labeled with a 6-carboxyfluorescein (FAM).
In this assay, two oligonucleotide probes for each polymorphic site were designed: one perfectly matching the wild type allele, the other perfectly matching the mutant allele.
In a teratoma formation assay, two iPSC lines from Δ133p53-overexpressing CRL-2097 fibroblands (Ci133-3 alongi133-5), along with two iPSC lines from control vector-transduced CRL-2097 (CiV1 and CiV4) and one from untransduced CRL-2097 (CiC1), were subcutaneously injected into NOD/SCID mice.
In the qualitative PCR assay, two PCR systems were established with the event-specific and specie-specific primers respectively, and the limit of detection (LOD) was 0.1% (approximates to 37 haploid genome copies).
For the reductase assay, two different types of reductase substrates were used.
For this assay, two cocktails were prepared as shown in Table 2, in two separate cuvettes and kept on ice.
In this assay, two distinct binding sites were noted for the 4-[18F]FEBZA, wIC50IC50 values of 1.8 nM and 1.3 μM.
For the implanted tumours' growth assay, two high-metastatic-potential and two low-metastatic-potential cells in 1 × 106/0.2 ml phosphate buffered saline were injected subcutaneously into the right flank of each mouse.
During the whole biological assay two resonance peaks per sensor (mode 10 and 11) with 3000 data points per peak were measured with a sampling rate of 5 × 106 samples/s.
