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Moreover, the sensitivity of FCS to molecular weight provides information on multimer size which is not captured by a typical immunoassay, potentially allowing an FCS-based assay to take the place of multiple tests.
Fit-for-purpose validation provides biomarker researchers with an approach that tailors the burden and stringency of proof required to validate an assay to take account of both the nature of technology utilised and the context in which the biomarker will be applied.
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Additionally, DEChN-F and DE-TO-ChN-F washed by distilled water were also assayed to take into account any influences from acetic acid.
To overcome this issue, we decided to carry out MTT assays, to take into consideration the heterogeneity in the cell number distribution while setting up the experiment.
This indicates that whole genome amplification after digestion in DMH and MMASS may introduce PCR bias; thus, it is important when using those two assays to take this into consideration for evaluating results.
As the rationale of including the control strains in the assay is to take into account the possible non-specific effects (e.g., toxicity) of the sample on bacterial bioluminescence, the high initial bioluminescence allowing more accurate measurement of the inhibition of bioluminescence, is certainly a bonus.
We also analyzed commercial and in-house assays separately, to take into account the potential differences between the assay techniques.
The aim of these assays was to take the effects of the juice matrix, especially of sugar inhibition at still optimal pH (adjusted to pH 5.5) into account (see Table 2 for juice composition).
To estimate killing rates from this assay, we need to take into account two complications.
We used a "whole-blood" assay in order to take into account the possible implication of plasma factors on TLR responses.
Nevertheless, while designing a new miR-Q assay, one has to take into account that an overlap of more than 3 nucleotides between RT6-'miRNA' and 'miRNA'-rev should be avoided because it results in high background of assays.
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