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Here, we use a new kind of wound healing assay, called a sticker assay, to explore the role of initial wound shape.
The present study was designed to establish a suitable assay to explore CCR2b receptor antagonists from the natural products of Artemisia rupetris and Leontopodium leontopodioides.
Finally, we used the rescue assay to explore whether the selective requirement for endogenous ActRIIA and BMPRII subunits in BMP7-evoked chemotaxis is absolute.
To assess the possibility that Pias3 employs Trim32 as its ubiquitin E3 ligase, we firstly utilized a GST pull-down assay to explore the potential association between Pias3 and Trim32.
In order to confirm the observation that inhibition of DNA-PK decreased rAAV replication in cells, we employed a previously developed in vitro replication assay to explore the role of DNA-PK and Ku70/80 [43], [43].
We next used the adult phagocytosis assay to explore whether hemocytic function changes with age.
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In this study we have used electron microscopy, flow cytometry and other assays to explore various sites of action shown by such compounds against Candida cells.
We also employed electrophysiological assays to explore the potential for acute post-translational modification of ion channels during patch clamp experiments.
Our study combines high resolution ion conductance measurements with biological susceptibility assays to explore β-lactam translocation properties through Omp36, a major porin of the MDR pathogen, E. aerogenes.
We did not observe a clear-cut association of FIP200 with the nuclei of the U-87MG or primary human MvECs, but we did not utilize biochemical assays to explore this possibility and cannot rule out a partial nuclear localization.
This concept was previously used in drop-based assays to explore gene expression and cellular responses [3] thus potentially adding functional information to the essentially descriptive large scale studies on genome, transcriptome and proteome performed within the emerging paradigm of Systems Biology [4].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com