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Addition benefits are shorter assay times and lowers costs, as well as reducing equipment and training requirements.
Experiments were designed to test the effects of stirring (0 600 rpm) during assays and the effects of varying the assay times (2 15 h).
Lateral flow assays (LFAs) have attracted interest due to their friendly user formats, short assay times, little interferences, low costs, and being easy by operated by non-specialized personnel.
This method provides a new avenue for high throughput screen of mycotoxins due to the advantages of simple instrument, low sample consumption, short assay times, and lower detection costs per assay.
Furthermore, since the assay has a Q10 for peroxyl radical-scavenging of about 3, elevation of the assay temperature from the standard 37 to 47°C significantly reduced the assay times.
Conventional pathogen detection methods, such as enzyme linked immunosorbent assay (ELISA), culturing or polymerase chain reaction (PCR) require long assay times, and complex and expensive instruments, which are not adaptable to point-of-care (POC) needs at resource-constrained as well as primary care settings.
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The highest concentration of enzyme used and assay time are indicated in the black wedges.
Without PCR the total assay time amounts to 27 min.
One cycle of assay time is less than 15 min.
An assay time of 32 min was achieved.
The total μPAD assay time was less than 30 s.
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