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As observed with the colony-forming assay, this effect on DNA damage was independent of disease status.
However, and as observed in the yeast-based assay, this effect was GAr-specific only for DXR, because this drug did not increase antigen presentation of Ova alone.
In the Boyden chamber assay this effect was present with and without concentration gradient of the cytokines indicating that basic mechanisms of cell migration like adhesion processes, MMP-synthesis and - activation as well as cytoskeletal reorganization may be negatively affected.
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Finally, a pre-stimulation culture period improved the sensitivity of the assay, however this effect may be both antigen and donor dependent.
There was variation in the magnitude of the effect observed using this assay, but this effect was robust and readily replicated across tissues.
Moreover, Gem promoted preferential mRNA translation as determined in a TK-ATF4 5′UTR-Luciferase reporter assay, and this effect was also reversed by ISRIB.
Furthermore, Gem enhanced the readout of a TK-ATF4 5′UTR-Luciferase reporter assay, and this effect was completely reversed by ISRIB, confirming that Gem-induced stress also acted to promote preferential translation of mRNAs.
MAN1 increases BMAL1 transcription in the HEK293 luciferase reporter assay but this effect was overshadowed by the presence of either RORα or REV-ERBα, and the impact of RORα and REV-ERBα on BMAL1 was not influenced by the presence of MAN1.
Dilution enhancement is a common problem with the LAL assay and this effect may be compounded in a cardiac bypass patient population due to changes in plasma composition during the course of, and following, the bypass procedure due to the use of cardioplegia solutions, crystalloids and hemodilution.
Because serum-free medium was used in this assay, this inhibitory effect could not be caused by targeting residual amounts of VEGF.
None of the other ABDs we assayed produced this effect.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com