Sentence examples for assay the viability from inspiring English sources

Objective: To validate a new combination of a viability assay and Fluorescence in Situ Hybridsation (FiSH) techniques using X- or Y-DNA probes to assay the viability of each single population within bidirectional mixed lymphocyte cultures (MLCs).

pIpa can tightly control the expression of the amber mutant GFP in mycobacteria growing inside the host cells, which makes this pIpa-induced reporter system a useful tool (in addition to shorter-lived fluorescent protein variants) to assay the viability of intracellular mycobacteria.

To assay the viability of B. burgdorferi, the SYBR Green I/PI assay or LIVE/DEAD BacLight bacterial viability assay was performed.

To assay the viability of the microbial cells during the invasion experiments, different concentrations of QS 50 and 100 μM, were added to E. coli suspensions (1 × 10 CFU/ml) in MEM following an overnight culture at 37°C.

To monitor the cytotoxic effect of the compounds selected from anti-EV71 screening assay, the viability of RD or Vero cells following 24 h of serially diluted compounds in the presence of 10% FBS and 0.5% DMSO treatment was determined in 96-well tissue culture plates using cell proliferation reagent WST-1 (Roche).

Thus, in addition to the endpoint WST-1 assay the viability of nonactivated and activated macrophages exposed to different Cd concentrations was determined using this method.

Quantitatively, we also assayed the viability of RIN-m5F cells encapsulated in PEG-based hydrogels using an AlamarBlue reagent as well as a CellTiter-Glo reagent.

To test this hypothesis we assayed the viability of hsf1−/− MEFs transfected with either an empty vector, wild-type HSF1 or the M161I, K178E, H179Y, L189P or L402P HSF1 mutants after exposure to a 42° heat shock for 2 h.

Presgraves (2003) performed a more sensitive screen by crossing D. melanogaster females with D. simulans males carrying the Lhr hybrid rescue mutation and assaying the viability of rescued F1 hybrid males (Presgraves 2003).

The BSK-H medium, which produced a high background for the BacLight viability assay, did not affect the SYBR Green I/PI assay, and the viability of B. burgdorferi cultures could be directly measured using a microtiter plate reader.

Consistently with the viability assays, the transfection of OLEO-TCII plasmid did not increase either cleaved Caspase-3 or propidium iodide uptake (Fig. 2B and 3).