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To quantify the relative levels of mRNA expression in the multiplex RT-PCR assay, the value of the GAPDH in each reaction was used as the baseline gene expression of that sample and relative value was calculated for the RASSF1A and BLU genes for each tumor and matched normal samples.
For ABTS radical cation decolorization assay, the value is 3.90±0.03 mmoL AAE/g fractions.
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In the RT-PCR assays, the values indicated represent the means ± S.E.
In the NADH APAD+, NADH HAR and NADH paraquat assays, the values observed are decreased moderately with respect to the WT values, and in most cases, the rates of H2O2 production were normal.
Although the KiPi values were consistent between bulk and rotation assays, the KiADP value determined from the bulk assay was lower than that determined in the rotation assay.
Along with the limited scope of assays, the translational value of the animal model must be considered as a potential limitation of the present study.
Compared the direct unamplified assay, the values obtained for the limit of detection for type IV collagenase was 0.96 ng mL−1, yielding about 6.5 times of magnitude improvement in sensitivity.
The mean (range) were 0.25 (0.19–0.32) µmol/L for control level 1 and 0.76 (0.57–0.95) µmol/L for level 2. In each assay, the values of the controls were in the range established by the manufacturer.
For each of the four respiratory pool assays, the mean values from CuSO4-untreated samples were then subtracted from the mean values of the CuSO4-treated samples.
Protein concentration was determined by Bradford assay and the value of MDA was normalized to mg protein used in the assay.
In this assay, the IC50 value of pregabalin was 89 nM, in accordance with reported values.
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