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A mass spectrometry method to assay the quality of protein in a tissue may provide a better method than an immunohistochemical stain.
To assay the quality of the purified plasmid library, a small amount was retransformed into E. coli XL1-Blue and plasmids of isolated clones (n = 14) were subjected to restriction analysis (Fig. 1B).
Before performing the microarray assay, the quality of the DNA was examined on a NanoDrop spectrophotometer (Thermo, Wilmington, USA).
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And to be honest, I'm not sure if we're even assaying the quality of the leadership with much deftness.
Basic metrics assaying the quality of generated data are provided for each dataset generated.
Prior to the QRT-PCR assays, the quality of the cDNA was assessed by PCR with gapdh-specific primers to test for genomic DNA contamination.
As we have learned more about the design, chemistry and kinetics of array assays, the quality of microarray data has improved dramatically.
Prior to the real-time RT-PCR assays, the quality of the cDNA was assessed by PCR with gene-specific primers for ubiquitin associated protein (UBA; AW508375) to test for genomic DNA contamination, as these primers amplify a larger fragment size from genomic DNA than from cDNA.
Preparation of standard curves with hESC genomic DNA revealed amplification efficiencies between 90 and 100% and R2 values of 0.99 for all assays (Fig. S3A), showing that the assays fulfilled the quality requirements for copy number determination on genomic DNA [31].
Thus, the FRET-based Atg4 assay has the quality to be successfully employed in HTS.
Due to the nature of IHC assay itself, the quality of IHC data depends heavily on the quality of the antibody.
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