For the biofilm assay, the materials containing attached bacteria were washed with sterile PBS and incubated in fresh LB broth for 24 h, 48 h, and 72 h at 37°C.
Annealed and strain-hardened copper are used as model materials to assay the physical consistency of the continuum crystal plasticity theory used in the simulations.
The same viral stock was used for all experimental infections, and dose verified by plaque assay using the material left over after infection.
We have further clarified the quantification of telomerase enzyme assays in the Materials and methods and Table 1.
In order to determine whether the 46 kDa band represented a phosphorylated isoform of the 41 kDa NDRG1 protein, we employed two different approaches, namely a dephosphorylation assay and the Phos-tag SDS/PAGE assay (see the Materials and methods section).
Problems occur at the level of the assay, the raw materials, or the sample.
We have now clearly explained the load for our binding assay in the Materials and methods.
We added text explaining the differences in the pumping rates among different experiments in 'Feeding assay' in the Materials and methods.
We have added a description of the actin co-sedimentation assay to the Materials and methods section of the revised manuscript.
