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A survey was designed to assay the level of guideline compliance in Florida.
HPLC was used to assay the level of ergosterol in all transgenic P. pastoris strains containing GuSQS1.
Cells from the same cultures were used to assay the level of expression of the StrepII-tagged integrases by western blots (Table 1).
The miniantibodies were analyzed by Western blotting under reducing and non reducing conditions and after treatment of the purified miniantibodies with glycosidase PNGaseF to assay the level of glycosylation.
We used periodate oxidation followed by polyadenylation, reverse transcription, and quantitative real-time PCR (RT-qPCR) to assay the level of miR-34a in intestinal RNA isolated from the mice.
Although within the detection limit of the assay, the level of contaminating plasmids was neglectable relative to measured copy numbers of total vector DNA (data not shown) and considered, therefore, to be without notable influence on measurements of total vector DNA.
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In this assay, the levels of ectopically expressed p53 in mammalian cells in the absence or presence of co-expressed E6 protein are evaluated by Western blotting.
Based on the cytokine array assay, the levels of GCP-2, GRO-α, ENA-78, IGFBP-3 and LAP secreted from CAFs were lower than NAFs (Figure 2A).
Interestingly, in this assay, the levels of cholesterol which have an inhibitory effect on Kir channels are well below those typically found in plasma membranes of mammalian cells (between 30 50% of total membrane lipids [2], [3]).
By ELISA assay, the levels of CCL21 in lung lysates and serum were elevated above baseline in the group vaccinated with LPS+rPorB and then challenged with LVS, as compared with unvaccinated unchallenged mice.
We therefore decided to assay the levels of pyridine dinucleotides in the leaves of wild type and transformant plants.
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