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We therefore devised an alternative way to assay the function of the Wnt gpi anchor in Wnt secretion.
There was no test to assay the function of lamin variants in vivo.
Furthermore, addition of ascorbic acid and TMPD to assay the function of complex IV revealed a smaller increased of oxygen consumption in MDA231 cells compared to BT474 cells.
In addition, these data help to interpret experiments that assay the function of T1 tRNATyr on the ribosome discussed in the next section.
To assay the function of set1/mll family members in planarian regeneration, we fed animals bacterially expressed dsRNA targeting each gene then amputated the animals anterior to the pharynx and observed them during regeneration.
All of the Ig-fold variants tested here caused dominant muscle defects, similar to laminopathies in humans, suggesting that Drosophila can be used to assay the function of novel sequence variants for their pathogenicity.
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To assay the functions of the sense-oriented genes, we fused V2 V1 to the IR, forming a TYLCV-derived 1,467-bp 1,467-bpn pDRinsertnd named in "pDRIVE.
To verify the potential cis-regulatory functions of CNEs that were absent in H. comes but present in other teleost genomes, we assayed the function of seven selected zebrafish CNEs that were uniquely absent in H. comes.
However, given the conservation of eve expression between D. melanogaster and C. capitata, and the success we and others [17], [18], [19], [20] have had in assaying the function of non-Drosophila dipteran enhancers in transgenic D. melanogaster embryos, we generated transgenic D. melanogaster lines for each tephritid eve CNS.
Previous studies have assayed the function of S303 and S307 phosphorylation in HSF1 regulation via in vitro phosphorylation experiments [22], in vivo using lexA/Gal4-HSF1 fusion proteins lacking the native HSF1 DNA binding domain [17], [18] or via overexpression of a S303A HSF1 mutants in mammalian cells expressing endogenous wild-type HSF1 [16].
Here, we assayed the function of the BLM homolog OsRecQl4 during DNA replication in rice via knockout mutants.
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