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b Caspase 3/7 activity assay: The data were expressed in fold change with respect to untreated control groups.
In this assay, the data from fluorescence spectrophotometer displayed a dose-dependent manner of CHIR99021, with the EC50 of around 206 nM, and the maximum effective concentration was 1 µM (Fig. 3g, 3i).
To determine if any significant differences existed between cultured PGCs and purified PGCs in the migration assay, the data were analyzed with the general linear model (PROC-GLM) of SAS software.
Although the male attractiveness induced by DA reduction did not depend on the high temperature, which was proved by our pharmacological assay, the data showed that temperature itself could also enhance male attractiveness for other males (CIwild-type31°C = 12.94±2.4 s.e.m.; CIwild-type25°C = 3.80±2.2 s.e.m.; p<0.001, Mann-Whitney Rank Sum test).
Since chain alignment leading to interchain disulfide bond formation is the crux of the chain-assembly assay, the data strongly suggest that there is an inability to create interchain disulfide pairing, and this kinetic blockade in the folding of the insulin chains (rather than instability of the native state) leads to enhanced cysteine thiol exposure that can promote intermolecular attack.
In the quantification of eye size in apoptosis assay, the data were analyzed with ANOVA.
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In many biological assays, such as colorimetric, fluorometric, or chemiluminescent assays, the data are a result of the mean response of the complete cell population.
TJ performed the genome walking, cloning, sequencing, reporter assays, the data analysis and drafted the manuscript.
For in vitro assays, the data were analyzed by ANOVA test and Student's unpaired t-test.
The entire 125I-labeled ligand binding experiment includes three parts: the radiolabeling, cell saturation binding assays and the data analysis.
AA designed the study, performed the immunoblot assays, analyzed the data and drafted the manuscript.
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